Bangladesh ousted prime minister Sheikh Hasina, her younger sister Sheikh Rehana and 69 others were accused in a murder over the killing of garment worker Md Fazlu.
He was killed during the student-led mass uprising in Mirpur-14 on August 5.
Suraiya, wife of the deceased, on Monday filed the case against Hasina, Rehana and 69 others with the Court of Dhaka Metropolitan Magistrate Md Saiful Islam.
After a hearing, the magistrate asked the Police Bureau of Investigation to submit a report following an investigation.
According to the complaint, Fazlu was shot in front of the police lines at Mirpur-14 at about 10:00am on August 5. Later, he was rushed to Max Medical College Hospital where doctors declared him dead.
Former home minister Asaduzzaman Khan Kamal and former state minister for industries Kamal Ahmed Majumder, among others, have been made accused in the case.